|Assumption of Risk
||You are REQUIRED to read
this, and you will have to sign a form in the laboratory
if you wish to remain in the class.
||You are required to bring
this manual to every laboratory session. If you prefer not
to print it, then you are allowed to use an electronic
copy. But in that case, it must be on a pad or laptop. A
cell phone is not large enough.
to Theoretical Drosophila
Do the assignment before you look at these. It's very important that you understand what we are doing and why we are doing it, and if you just look at the answers instead of trying the assignment first, you'll just cheat yourself.
||Figure out the crosses on your own, then check to make sure you set up the crosses correctly.|
|Bar Eye Flies (Exercise
||This is required reading
for Laboratory Four. The information will be treated on
|GMO (Exercise Six)
This document is for the GMO laboratory periods. The quizzes will treat this material, but also be sure to read the information in the book, as indicated in the Laboratory Manual.
(Exercise Seven, Part One)
This is for the pGLO experiment. The quiz will treat this information.
|GFP Purification (Exercise Seven, Part Two)||
The quiz will treat this information.There is one protocol modification. On page 34, eliminate the freeze/thaw step. Instead, incubate with lysozyme at room temperature with intermittent vortexing for 15 minutes.
|GFP SDS-PAGE (Exercise Seven, Part Three)||
The quiz will treat this information.
||This should be printed. An
electronic version will not work.
Enzyme Mapping (Exercise Eight)
||Do the exercise in your
notebook. It will be checked as part of laboratory
notebook grading #2. This exercise is taken from Carolina
Read Chapter 18 before you do this laboratory exercise. We do not have a laboratory session set aside for this exercise. You should be working on this exercise during laboratory down-times, such as when we run gels or when we get out early.
This can be used as a reference for your Drosophila paper.